Critical Evaluation of Two Commercial Biocontrol Agents for Their Efficacy against B. cinerea under In Vitro and In Vivo Conditions in Relation to Different Abiotic Factors

The study evaluated the dose–response relationship of two commercial microbial biocontrol agents, Bacillus subtilis and Gliocladium catenulatum, against Botrytis cinerea both in vitro vivo. Inoculum doses, formulation, temperature foliar leaf part all affected control achieved by BCAs. In competition assays on modified PDA plates tested a range BCA doses (log10 3–10 CFUs or spores/droplet) at 4, 10 20 °C development B. colonies. was influenced formulation temperature. vivo studies lettuce plants semi-commercial greenhouses examined dose 5–9 spores/mL) for controlling with high inoculum 6 spores/mL). Leaf disc showed that parts sampled. These results suggest between specific pathogen will be significantly environmental conditions, plant phyllosplane tissue.